Reversible transgenesis that leaves no trace behind

One of the advantages of the PiggyBac Transposon System* is the reversibility of the integration event. With the Excision-only PiggyBac Transposase, delivered by transient transfection of the Exicison-only PiggyBac Transposase Expression Vector, you can remove any DNA integrated into the genome using the PiggyBac Transposon System. After removal of the PiggyBac insert, the genomic DNA is restored to the original sequence with no residual PiggyBac sequences left behind, resulting in a truly footprint-free removal.

Both the Excision-only PiggyBac Transposase and the Super PiggyBac Transposase (Cat.# PB210PA-1) recognize transposon-specific inverted terminal repeats (ITRs). While the Super PiggyBac Transposase has both excision and integration activities that enable the enzyme to mediate removal of the ITRs and intervening DNA from one location (the vector) and insert that DNA fragment into another location (the genome), the Excision-only PiggyBac Transposase only has the excision activity. Thus, the Excision-only PiggyBac Transposase can be used to remove DNA segments from the genome that are bordered by the PiggyBac transposon ITRs.

With the PiggyBac Transposon System, you can:

• Make transgenic cell lines with a single transfection
• Integrate multiple PiggyBac Vectors in a single transfection
• Insert an expression cassette into human, mouse, and rat cells
• Deliver virtually any-sized DNA insert, from 10 – 100 kb
• Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
• Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to evaluate the technology.

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.

美国SBI代理 System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒 美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1